ABSTRACT
<p><b>OBJECTIVE</b>A quantitative method was developed for the determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC.</p><p><b>METHOD</b>The samples were separated by Alltima C18 column (4.6 mm x 250 mm, 5 microm) using methanol--0.05% phosphoric acid (65:35) as a mobile phase, flow rate was 1.0 mL x min(-1) and wavelength was set at 202 nm.</p><p><b>RESULT</b>Tenuifolin was detected in both Chinese preparations. The number of theoretical plates calculated by tenuifolin peak was 2 500. The regression equation of tenuifolin was Y = 5.239 x 10(6) X-6.247 x 10(5) (r = 0.9994) and the liner range was 10-500 g x mL(-1). The average recovery of tenuifolin was 97.5% (RSD less than 3.0%). The LOD of tenuifolin was 5.50 g x mL(-1).</p><p><b>CONCLUSION</b>The method is sensitive, rapid and accurate.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Codonopsis , Chemistry , Diterpenes, Kaurane , Drug Combinations , Drugs, Chinese Herbal , Chemistry , Plants, Medicinal , Chemistry , Polygala , Chemistry , Reproducibility of Results , Salvia miltiorrhiza , Chemistry , Saponins , TriterpenesABSTRACT
<p><b>OBJECTIVE</b>To recognize changes in the contents of ingredients of Andrographis Tablet in the process of production.</p><p><b>METHOD</b>Adopting TLCS, TLC, HPLC to detect effective contents of ingredients which are produced in every stage of process of Andrographis Table's production.</p><p><b>RESULT</b>Handling with the fresh Herba Andrographis according to current pharmacopeoia's technology, it showed that only dehyandrographolide can be detected. It indicated that the main factor that leads to chemical change is the heating process in the process of production.</p><p><b>CONCLUSION</b>Avoiding heating treatment or reducing heating treatment time is the main factor to protect the effective ingredients.</p>